Protocol 41 Tissue samples and RNA isolation

Thirty-five kidney tumors (13 ccRCC, 13 pRCC, 9 chRCC), which belonged to a larger study (19) were processed by standard pathology. Immediately after surgery, tumor pieces were subjected to routine histopathological examination as described previously (19). Other tumor pieces were snap-frozen in liquid nitrogen and stored at -80°C. Following homogenization with a Micro-Dismembrator S (Braun Biotech, Melsungen, Germany), total cellular RNA was isolated by the Trizol method (TriFast, peqlab, Erlangen, Germany). RNA quality was checked with the Agilent 2100 bioanalyzer (Agilent Technologies GmbH, Waldbronn, Germany). Only high-quality RNA (28S/18S rRNA and E260/E 280 ratios close to 2) was used for the experiments.

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