Protocol 44 Signal quantification and data analysis

Arrays were scanned with the GenePix 4000B microarray scanner (Axon Instruments Inc., Union City, CA), and spots were quantified using Arrayvision 6.0 software (Imaging Research Inc., St. Catharines, Ontario, Canada). Intensity values for duplicate spots of each cDNA clone were averaged. Background-corrected intensity values were normalized and transformed to generalized log-ratios through the VSN method (20) (see also Chapter 17). All analyses were performed using R (, and Bioconductor packages (www.

Gene expression analysis of differentiating neural progenitor cells - a time course study

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