Scattered light scan slide quality control

Microarray spots, which have no label, can be detected for quality reason without using an indicator dye, if they contain salt crystals (SSC in spot ting buffer). Any light will be scattered at these spot crystals and can be detected by using the delivered absorbance filter of the LS for scanning. An automated image analysis for local and global background, spot density, spot morphology, and spot position, can be applied. A report of 'passed or failed' spots and slides by using appropriate (customized) statistics means that low quality slides can be rejected from hybridization and false negative and positive spots can be discarded from the analysis and re-evaluated.

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