Selective beam splitting dicroics

To enable dual color simultaneous detection with LS, a set of two laser beams is synchronized and focused on the same area. The generated fluorescence spectra from different dyes are collected by a lens and mirror system. The mix of two dye spectra is subsequently discriminated by different dicroics (wavelength-selective mirrors with a discrimination line at 575 or 625 nm). To discriminate between red and green the LS uses a 625-nm dicroic and to discriminate blue from yellow and red a 575-nm dicroic is used (Figure 16.2).

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