cDNA clone master plate DNA preparation * PCR

Preparation of cDNA insert Microarray Spotter

Test plant material Control plant material

{ Isolation of total RNA J


Reverse transcription Preparation of Cy3- and Cy5-labeled cDNA targets

Print on glass slide cDNA microarray

Cy3 Cy3

cDNA microarray

Print on glass slide

Cy3 Cy3

Mixing of the cDNA targets


Detection of fluorescence using scanner

Conversion of image data to numerical data Data analysis

Identification of up- or down-regulated genes Searching for novel c/'s-acting elements Formulation of new hypotheses

Figure 3.1.

Overall strategy for cDNA microarray analysis

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