Test yourself

1__are enzymes that cut DNA at specific_ sequences in the__backbone.

2 Joining together DNA fragments from different sources produces a_


3 DNA fragments with__tend to join together because of their complementary single-stranded overhangs.

4 In order to gain access to a host cell and be replicated, a fragment of DNA must be taken up by a_molecule.

5__enable us to detect which host cells have been transformed. A commonly used example is resistance to_ . By inserting foreign DNA at this point, recombinants can be detected by_

6 Another name for a polylinker is a___.

7 A short sequence of labelled single-stranded DNA used to locate a specific target sequence is known as a__

8 The removable central section of the phage Lambda genome is known as a _fragment.

9 Bacteriophage_is a useful vector if DNA is required in a_

10 Cloning vectors combining features of plasmids and phage Lambda are called

_vectors incorporate foreign DNA at a site that is surrounded by essential signal sequences.

12 E. coli is not a suitable host for the expression of complex eucaryotic proteins because it lacks the molecular machinery to carry out_modifications such as__

13 _DNA contains only those sequences that are involved in coding for a polypeptide product.

14 Yeast plasmids are based on a naturally occurring sequence called the

15 _vectors are able to replicate in both_and_cells.

16 A virus found to be useful in the expression of eucaryotic genes is the __It is grown in cultured_cells.

17 The soil bacterium A. tumefaciens contains a plasmid called_, which causes crown gall disease. Part of it, the_ , integrates into host chromosomes.

18 Genes from Bacillus thuringiensis coding for_have been introduced into plant cells, making them_resistant.

19 The polymerase chain reaction (PCR) comprises repeated cycles of the following three steps:_,_of primers and_by_

DNA polymerase.

20 PCR results in an_increase in the number of copies of the target sequence.

Part V

Control of Microorganisms

Was this article helpful?

0 0
How To Win Your War Against Bronchitis

How To Win Your War Against Bronchitis

Sick And Tired Of Your Constant Cough? Is Your Bad Immune System Leading You To The Path Of Fever And Sore Chest? You Sure Have A Reason To Panic BronchitisThere Is Always A Way Out And, This Is It Finally Discover Some Of The Most Effective Tips That Can Curb Bronchitis, And Its Repeated Bouts Learn How To Keep The Chronic Cough, And Sore Chest Away Breathe Free, And Feel The Whiff Of Fresh Air, With No Hassles

Get My Free Ebook

Post a comment