Snp Typing Using The Lamp Method

To detect a single nucleotide difference by LAMP-based SNP typing, both FIP and BIP were designed to contain SNP nucleotides on the target sequence at each 5' end. Figure 3A shows the basic principles of the LAMP cycling reaction with the WT primer set, using WT DNA Fig. 3A, upper and MUT DNA Fig. 3A, lower as templates. When LAMP reaction using the WT primer set is carried out with WT DNA as the template, a dumbbelllike structure is generated with both ends being overlapped by self-annealing...