A1555G Mutation In The 12S rRna Gene

A1555G mutation can induce hearing loss in the presence or absence of exposure to aminoglycoside antibiotics. It was homoplasmic in almost all of the reported cases, although a low level of heteroplasmy has been reported.

There are currently available polymerase chain reaction (PCR)-based screening tests for this mutation. Following are the details of a simple PCR-restriction fragment length polymorphism (RFLP) test:[1]

Primers

Forward 5'-CCGCCATCTTCAGCAAACCCT-3'. Reverse 5'-TGAAGTCTTAGCATGTACTGCTCG-3'.

Following amplification, PCR products are digested with the Alw26I restriction enzyme, of which one cleavage site is abolished when A1555G is present.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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