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cDNA synthesis cDNA or genomic DNA

Synthesis of primers with 5' complementary tails

Two separate PCR amplifications

Denaturation and reannealing

Extension and PCR amplification of DNA competitor

Cloning in a plasmid vector and in vitro run-off transcription of RNA competitor

Fig. 1 Construction of competitor templates for competitive PCR or RT-PCR. The figure describes all the steps necessary to the synthesis of an RNA competitor. Steps from a to e represent the procedure to obtain a DNA competitor template.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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