Absolute Quantification

''Absolute'' quantification is not really absolute, but is relative to an external standard curve.[2] A standard dilution series with a known concentration of initial target

Instruments used for real-time PCR use: 1) an excitation light source, to excite the fluorophores; 2) a detector, to register photon emissions that are proportional to the concentration of the amplification product being measured; and 3) a software, which allows analyses of the data.

Fluorescence emission data are collected from each tube and the levels of background fluorescence detected by the fluorimeter are established. Platform-specific algorithms are used to define a fluorescence threshold. Finally, the algorithm searches the data from each sample for a point that exceeds the baseline. The cycle at which this point occurs is defined as Ct (Fig. 2) and is used to calculate the amount of template present at the beginning of the reaction.[22]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment