Advantages And Disadvantages Of Fish Banding Methods

FISH banding approaches such as the chromosome bar code technique using YACs, region-specific humanhamster somatic cell hybrids, or nonoverlapping microdissection libraries (SCAN) have, per definition, the disadvantage that unstained and noninformative gaps are left along the chromosome. Such gaps can cause problems, as breakpoints within the unstained gaps cannot exactly be described. Conversely, techniques based on locus-specific probes would theoretically provide the advantage that chromosomal breakpoints could be defined very exactly by the corresponding breakpoint-spanning or flanking clones. However, the coverage of the human genome by nonchimeric clones seems to be presently too low for such a molecular cytogenetic approach (Liehr et al., unpublished data). The IPM-FISH approach, Rx-FISH technique, Rx-FISH combined with somatic cell hybrids, and the MCB method provide the advantage of leaving no noninformative gaps.

Only FISH banding probe sets (1), (4), and (5) are finished for the whole human genome and can be applied to achieve comprehensive information in one single hybridization step. Concerning banding resolution, MCB has the highest and most flexible one. Additionally, according to the question to be studied, it can be chosen if only selected chromosomes or the whole genome shall be hybridized.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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