Advantages With Mlpa

1. One technique can be used for many applications. The only difference between different assays is the probe mix used. Hybridization, ligation, and PCR reagents and conditions are identical for the various applications.

2. Multiplex: One reaction provides information on up to 45 targets. For most applications, this is sufficient to answer the specific question asked by the physician.

3. Easy to perform. Large numbers of samples, e.g., 96, can be tested simultaneously.

4. Sensitive. Only 20 ng of human DNA is required. Results do not depend on the amount of sample DNA used.

5. Reproducible. MLPA can detect small differences in copy number such as three vs. two copies of a gene sequence in a complex mixture like the human genome.

6. Requires a target sequence of only 60 nucleotides. Can therefore be used to detect deletions or duplications of a single exon and permits use of DNA of poor quality such as DNA extracted from formaldehyde-treated, paraffin-embedded tissues.

7. Sequences that differ in only a single nucleotide can be distinguished.

8. Low investment. The equipment necessary for MLPA, a thermocycler and DNA sequencing electrophoresis equipment, is present in most molecular biology laboratories. Capillary electrophoresis is preferred but MLPA has also been successfully performed on the older ABI 373 and 377 slab gels.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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