Advantages

Colony PCR is a whole-cell-based assay, and thus will save a considerable amount of time, labor, and chemical reagents. The possibility of experimental errors or contamination is also reduced because DNA purification is not required.[3] A comparison study with two DNA extraction methods using mycobacterial isolates indicated that the heating method is more sensitive and no less specific than a conventional chemical method (phenolchloroform). The loss of DNA during purification and the presence of inhibitory substances are the most likely explanations for the lowered PCR sensitivity results obtained via the chemical method.[8,9] PCR is a very sensitive and rapid method of detecting specific DNA sequences in a wide variety of samples. The most common problem is contamination of reagents with target DNA. However, colony PCR will be performed on bacterial colonies directly obtained from broth culture or plates, which will lower the opportunity of contamination resulting from DNA manipulation or extraction. In addition, no detergents or proteases are used to lyse the bacterial cells, which is also advantageous because these may interfere with the PCR reaction.[3]

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Healthy Chemistry For Optimal Health

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