Aflp Analysis

AFLP is a PCR-based technique able to provide high-throughput DNA fingerprinting for rapid and accurate screening of genetic diversity, which was correctly originally described as able to ''bridge the gap between genetic and physical maps.''[1] The method comprises of a restriction-enzymatic digestion of genomic DNA followed by ligation of double-stranded oligonucleotide adapters that are complimentary to the restriction sites at the ends of the DNA fragments. The ligated ends are designed not to regenerate the restriction sites, and because the adapters are not phosphorylated, adapter-to-adapter ligation is prevented. Because the adaptors are of known DNA sequences, subsequent PCR amplification under high stringency is performed using primers that have exact complementarity with the template DNA generated by the adaptors. Patterns of amplified product differing in size constitute the final output of the process (Fig. 1). Despite the name, and despite its similarity with restriction fragment length polymorphism, AFLP detects the presence or absence of restriction fragments rather than their length polymorphism.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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