Antibody Validation

A limiting factor for the successful application of this reverse phase protein microarray technology for monitoring phosphorylation events is the availability of specific, high-affinity antibodies.[6,12] DNA probes have been manufactured with known specific affinity constants. On the other hand, antibodies, aptamers, and ligands utilized for protein microarrays cannot be manufactured with predictable affinity or specificity. Reproducible protein microarray analysis relies on antibodies that are specific for the protein of interest. Validation of antibodies via Western blotting is typically performed with a complex biologic sample similar to the sample that will be used on the microarray. A validated antibody should reveal a single, specific band at the expected molecular weight, thus indicating its specificity.

Table 1 Comparison of contact and noncontact microarray printing technologies
Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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