Application for Other Organisms

This new RFLP approach has been applied in a number of situations. Shortridge et al.[20] used the RFLP of the ureC gene to demonstrate the genetic diversity of Helicobacter pylori strains in the United States. The 16S, 23S, and 16S-23S spacer regions have also been used as targets for locus-specific RFLP.[21] In this variation of ribotyping, the ribosomal DNA is amplified and subjected to digestion with restriction enzyme, and the DNA fragments are visualized following separation by gel electrophoresis avoiding the need for Southern blotting.

Gene-specific probes have been used to subtype

Brucella species,[22] Legionella pneumophila,[23] and

Pseudomonas aerugenosa.[24] Furthermore, ribotyping has been applied successfully in many studies to differentiate bacterial strains.[25,26] Other applications of locus-specific RFLP found place in epidemiological studies of hepatitis C virus (HCV). By this technique, the virus can be subtyped into six major genetical groups.[27] The RFLP of the 5' untranslated region has facilitated studies of the geographical distribution of viral genotypes and natural history of the disease.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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