Application for Other Organisms

Ribotyping is the most versatile and the most widely used strategy of Southern blot analysis of bacterial genome polymorphism. The evolutionary conservation of ribo-

somal RNA makes it applicable as a universal bacterial probe. Many important pathogens, including Enterobac-teriaceae, Listeria, Pseudomonas spp., and staphylococci have more than five ribosomal operons and thus produce ribotype patterns of 5 to 15 bands.[17] Ribotyping is a robust method that exhibits excellent reproducibility and stability, during the course of the outbreaks. It is commercially available and fully automated and well standardized. However, its discriminatory power is only moderate, and this is related to the fact that ribosomal operons cover less than 0.1% of chromosomal length and tend to cluster in one particular region of the genome. Discrimination of ribotyping depends on the species and on the choice and number of restriction endonucleases used. No consensus has been achieved on optimal procedure and no general rules are available for interpretation of technically problematic results, such as weakly hybridizing fragments.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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