The increasing utility of real-time RT-PCR promises a paradigm shift in molecular clinical diagnostics. Its ability to detect the nucleic acid of a pathogen allows it to identify the actual causes of a disease, as opposed to merely detecting its symptoms. Alterations in mRNA expression profiles are associated with a tissue's reaction to pathological states or drug treatments, and are likely to prove useful for more accurate postoperative staging of cancer patients. Its speed, simplicity, specificity, and sensitivity make this technique ideally suited for this task, making it a cost-effective and time-efficient assay that could become part of a routine protocol of specimen processing.
However, it is important to be aware that issues such as sample processing, assay standardization, and repro-ducibility, as well as the use of appropriate diagnostic controls, remain to be resolved before real-time RT-PCR can become a realistic practical diagnostic assay. Furthermore, because data interpretation remains highly subjective, there is a need for strict quality control of the reported results to achieve a consistent, standard, and valid diagnosis based on real-time RT-PCR.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.