Applications in Quantitative PCR

There are a wide variety of tests for the quantitative estimation of viruses. New methods to quantitate small amounts of DNA polyomavirus[14] and CMV[15] in clinical specimens were described based on nPCR. The method, a nested competitive polymerase chain reaction (ncPCR), is able to quantitate between 10 and 105-106 copies per tube of virus DNA and showed good reproducibility when clinical samples were analyzed. Throughout the whole procedure, an internal standard (mimic) competes for the primers with the target DNA. A solution containing 2 x 1010 DNA copies/ml is used as target sequence to generate a standard curve. Signals of both bands are analyzed by computerized densitometry. The original number of DNA copies present in clinical specimens is determined by interpolating the logarithmic signal ratio of amplified products into the standard curve.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment