TMAs are optimally suited for large-scale expression profiling projects. Automation of the interpretation is the absolute key issue for such projects. In principle, TMAs are highly suited for automated analysis. The greatest difficulty for automated tissue analysis, the selection of an appropriate tissue area, has been solved to a large extent during TMA manufacturing. Several systems enabling automated TMA analysis have recently been introduced. Some of them utilize fluorescent dyes for immunostaining because of the better dynamic range compared to peroxidase-based systems and the potential for multicolor analyses. However, fluorescence-based immunostaining is limited by the complex staining protocols for multicolor applications and the difficult manual reevaluation of questionable staining results. Most commercial products for automated TMA analysis, therefore, concentrate on conventionally immunostained TMA sections. These are generally based on an automated microscope with a charge-coupled device (CCD) camera. An overview image is used to identify the localization of each tissue spot in the TMA for subsequent high-resolution scanning. Studies have shown, that such ''low-tech'' solutions quantifying the total signal intensity per TMA spot are easy to establish and surprisingly efficient for identification of clinico-pathological associations.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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