B

Add dilutions of competitor for target gene ratio=1.06 Concentration=9500molecules/5/í 1

Add dilutions of competitor for single copy reference gene

20 molecules x 10

Add dilutions of competitor for target gene

ratio=1.06 Concentration=9500molecules/5/í 1

ratio=0.4 Tatget gene n. copies=12

Fig. 2 Example of competitive PCR assay. The assay is performed in two-step (A and B) competitive PCR. In step A, the exact DNA concentration of each samples is accurately measured by reference to a single copy reference gene (i.e., b-globin), while in step B, the exact number of target gene copies was determined. In both PCR steps, we added three dilutions of DNA competitor (single copy reference gene competitor or target gene competitor). The competitor/genomic ratio was evaluated after acrylamide gel electrophoresis of PCR products.

ratio=0.4 Tatget gene n. copies=12

Fig. 2 Example of competitive PCR assay. The assay is performed in two-step (A and B) competitive PCR. In step A, the exact DNA concentration of each samples is accurately measured by reference to a single copy reference gene (i.e., b-globin), while in step B, the exact number of target gene copies was determined. In both PCR steps, we added three dilutions of DNA competitor (single copy reference gene competitor or target gene competitor). The competitor/genomic ratio was evaluated after acrylamide gel electrophoresis of PCR products.

Add dilutions of competitor

Add dilutions of competitor

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment