The Invader technology is based on the ability of a Cleavase® enzyme to recognize and cleave a specific nucleic acid structure generated by an overlap of two oligonucleotides (oligos)—the Invader oligo and the primary probe—on the nucleic acid target. Cleavase enzymes are a family of naturally occurring as well as engineered thermophilic structure-specific 5' endonu-cleases.[1] Cleavase enzymes for use in DNA assays are derived from members of the flap endonuclease (FEN-1) family, typically found in thermophilic archaebacteria.[1] Invader RNA assays use Cleavase enzymes that are engineered from the 5'-exonuclease domain of DNA polymerase I of thermophilic eubacteria.[2] The optimal substrate for these nucleases is composed of distinct upstream, downstream, and template strands, which mimic the replication fork formed during displacement synthesis. The enzymes cleave at the 5' end of the downstream strand between the first 2 base pairs and remove the single-stranded 5' arm or flap.[1]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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