Biological Relevance

Biopsies contain a range of different cell types—a problem exacerbated in heterogeneous tumor samples that include normal and inflammatory cells as well as diversely evolved cell populations. In addition, normal cells adjacent to a tumor may be phenotypically normal but genotypically abnormal, or exhibit altered gene expression profiles because of their proximity to the tumor.[37] Hence expression profiling of such biopsies provides a composite of the whole population, and this may result in the masking of the expression profile of a specific cell type, or it may be ascribed to and dismissed as illegitimate transcription.[38]

Laser capture microdissection (LCM) is useful for accurate expression profiling from such biopsies[39] and has become a powerful technique for extracting pure subpopulations of cells from heterogeneous in vivo cell samples for detailed molecular analysis.[40] Isolation of

RNA from such small samples is possible, and mRNA expression levels can be accurately and reproducibly quantified,1-41-1 even from archival paraffin-embedded tissue specimens1-42-1 and after immunohistochemical staining.[43]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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