Bio Robot Workstations

The BioRobot EZ1 workstation (Qiagen, Hilden, Germany) purifies nucleic acids from many clinically relevant specimens; one to six samples can be processed within 15 min of time. This rapid purification is feasible using a magnetic particle technology: nucleic acids in sample lysates are isolated in one step through their binding to the silica surface of magnetic particles. The automated protocols for nucleic acid purification are provided on special preprogrammed cards.

The BioRobot M48/M96 workstations run the identical magnetic particle technology, but the pipet tips function as reaction chambers. However, up to 48/96 samples can be processed per run in increments of six samples. The pipettor head contains six syringe pumps, which operate simultaneously to allow aspiration or dispensing of volumes between 25 and 1000 p.L. The workstation uses UV light to decontaminate surfaces in-between runs.

Finally, the BioRobot 9604 workstation allows automated nucleic acid purification for up to 2 x 96 samples in parallel.

Xu et al.[1] describe an automated assay for the extraction of HIV RNA by using the BioRobot 9604. They conclude that this procedure yields comparable results to a manual RNA extraction method with improved precision. The assay was found to be linear down to 50 copies.

For the extraction of hepatitis C virus RNA, it was shown that it is possible to achieve a detection level of 12.8 IU/mL (95% confidence). Cross-contamination studies have confirmed that the use of the BioRobot 9604 does not pose a detectable contamination risk.[2]

For hepatitis B virus detection, Mitsunaga et al. describe that it was possible to quantify DNA in all samples extracted by the BioRobot 9604 which contained more than 500 genome equivalents/mL. Extraction of 96 samples could be completed within 2 hr.[3]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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