Body Of Text HyBeacons

HyBeacon probes are target-specific linear oligonucleotides with fluorescent dyes attached to internal nucleo-

tides.[1-3] Probes emit significantly greater amounts of fluorescence when hybridized to complementary target sequences than were single-stranded. HyBeacon probes do not possess quencher moieties attached to the oligo-nucleotide and the mechanism of target detection does not rely on probe secondary structures or enzymatic diges-tion.[1] The inherent fluorescence-quenching properties of DNA are responsible for the lower levels of probe emission in the single-stranded state, and hybridization to complementary targets directly relieves a large proportion of that quenching. This phenomenon enables specific target detection and reliable discrimination of DNA sequences. HyBeacon functionality does not appear to be dependent on nucleotide sequence or the location of gua-nosine residues within probe and target as reported for other single-labeled technologies such as Simple probes, LUX primers, and Smart probes.[4-6]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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