when the internal positive control does not detect any inhibition in the PCR reaction, the presence of foreign DNA or nontarget DNA could still have interfered with the performance of the PCR, causing a drop in the sensitivity of the test or even leading to a false negative result when it is applied to samples with few target genes present.[10,14] Validations of PCR specificity are recommended, and may be performed with restriction fragment length polymorphism (RFLP) analysis, PCR product sequencing, or Southern blot hybridization.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.