Capillaries

Glass capillaries have the best optical properties for fluorescence measurements. They also have a large surface-to-volume ratio, leading to fast temperature ramping rates as well as being highly suitable as fluorescence measurement vessels. The high surface-to-volume ratio also increases the adsorption of reaction components to the glass walls, and protocols intended for plastic containers may need reoptimization. LightCycler capillaries have an outer diameter of 1.5 mm and a length of 3 cm, and can hold sample volumes of 5-20 p.L.[8] The new LightCycler 2.0 instrument can also use new 100mL capillaries in a special sample carousel. The shape of the capillaries concentrates the fluorescent signal to the tip, where emitted light is collected. The capillary has three parts: the actual capillary, a sample deposit chamber from where the reaction mix is centrifuged into the capillary, and a plastic cover to seal the capillary. Glass capillaries are rather expensive and containers are a major cost component of each PCR reaction run. The capillaries are also fragile and may break during handling.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment