Capillary Electrophoresis

Capillary electrophoresis has proven to be a versatile technique for analyzing a wide range of analytes. Variations include 1) open tubular or capillary zone electrophoresis (CZE), 2) capillary gel electrophoresis (CGE),

3) micellar electrokinetic chromatography, 4) capillary isoelectric focusing, 5) capillary isotacophoresis, and 6) capillary electrochromatography (CEC).[1-3] For the purpose of this overview, CGE will be described to illustrate its utility in DNA analysis with a brief comment on other analytes. Capillary zone electrophore-sis is used for the analysis of a wide range of charged simple organic molecules, inorganic ions, peptides, and proteins. Capillary gel electrophoresis is the method of choice for sizing and sequence analysis in the DNA diagnostic laboratory.

A simplified diagrammatic representation of CGE is given in Fig. 1. A constraint with the traditional slab gel electrophoresis is the heat generated by the applied voltage, which is limited to 5-40 V/cm. In contrast, a capillary is more effective in dissipating heat, thereby allowing higher voltages (up to 300 V/cm). This leads to shorter running times and sharper bands. The migration of an analyte is influenced by size, charge, and unique to CGE, is the electroosmotic flow (EOF). Only picogram amounts need to be loaded as the detection system can use a laser-CCD camera system. Software will allow the appropriate loading and running conditions to be selected and is also used for analysis and interpretation of the results.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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