Clinically Relevant Pilot Applications

The practical RCA potential to identify nucleic acid targets, antibodies, and antigens in clinical samples has recently been demonstrated in several feasibility studies. Specifically, the RCA-based protocol for an automated scoring of single nucleotide polymorphisms (SNPs) in a set of human genomic DNA samples with the nanogram sensitivity was developed.[15,20] Highly sensitive multiplex detection of hotspot somatic mutations present at very low abundance was also reported.[21] The RCA capability for pathogen diagnosis was convincingly proved by comparative study of RCA vs. PCR and ligase chain reaction (LCR) in detection of Chlamydia trachomatis in cervical specimens.[22] The workability of immuno-RCA for identification of allergen-specific immunoglobulins in samples from patients was shown in a microarray format.[23] In addition, RCA has been adapted to immunohistochemistry, flow cytometry, and in situ hybridization to significantly improve their sensitivity without compromising cellular and tissue morphology.1-24,25-1 RCA-mediated multiplex profiling of cytokines on microarrays with femtomolar sensitivity offers an advantageous approach for proteomic surveys.[16] All these pilot applications establish the firm basis for future clinical use of RCA methodology in a range of diagnostic applications, enabling the integration of genomic and proteomic information into cell- and tissue-based tests.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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