Competitive Hybridization

As hybridization of longer PCR products to microspheres results in significant cross-talk and decreased sensitivity, a different assay format, the competitive hybridization, was applied. With this technique, allele-specific, fluorescently labeled oligonucleotides (reporter oligonucleotides) are hybridized to the target amplicon. Afterwards, micro-spheres coated with oligonucleotides complementary to the reporter oligonucleotides are added and capture the remaining nonhybridized oligonucleotides. A decrease in fluorescence intensity compared to the negative control (inhibition of fluorescence) will report a positive test result. This assay format was used, for example, for a multiplexed hybridization assay to perform HLA-DQA1 tissue typing of PCR-amplified human genomic DNA.[7]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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