Conclusion

The risk of emergence of HSV strains resistant to ACV remains a major concern because of the increase in the number of immunocompromised patients and in the use of antiviral drugs, both for prophylactic and therapeutic treatments of herpesvirus infections. So far, even if phenotypic tests are relevant to detect resistant strains, they are time consuming and delay the change of antiviral treatment according to in vitro susceptibility. Therefore it is important to develop new tests based on molecular biology techniques to allow the detection of resistant viruses more rapidly. Because of the numerous mutations reported in resistant strains, both in TK and DNA polymerase genes, and because of the high degree of polymorphism, specially in the TK gene, a complete database of the mutations associated or not with resistance to antiviral drugs has to be established for the interpretation of TK and DNA polymerase sequencing results in new isolates. Genotypic tests based on PCR associated with sequencing would be performed directly on clinical samples preventing the delay of virus isolation by culture. The development of microarrays applied to HSV could constitute a further improvement in the detection of HSV resistance. These new assays may help clinicians in the management of antiviral treatment of HSV infections.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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