Conclusion

PCR-CLEIA allows rapid, specific, and sensitive detection of target DNA or RNA sequences. The use of chemiluminescence as a detection system provides optimal performance in terms of sensitivity and linear range of the method, and increases rapidity with respect to conventional spectrophotometric detection. Furthermore, chemiluminescence allows the use of innovative assay formats, such as multianalyte or high-density microtiter plate, thus further enhancing throughput of the method.

Future trends of this technique involve the development of miniaturized and fully automated assays, in particular the use of microarray and microchip technologies, which allow the execution of numerous multi-analyte assays in very small sample and reagent volumes and short times. However, at present, these systems are mostly applied to screening purposes because quantification accuracy of each microspot is hampered by the absence of physical separation and cross-talk phenomena.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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