Most of these PCR methods described here cannot be used for direct detection from clinical samples such as blood or stool. One has to isolate the causative agent by ordinary culture methods using SS agar or Maconkey agar plates, then apply several colonies on selective media to the PCR identification system. Thus, it takes 2 or 3 working days to get a final result.

The PCR methods described here may make it possible to detect and/or identify clinically important Salmonella serovars, such as S. Typhi, S. Paratyphi A, S. Enteritidis, and S. Typhimurium, within a few working days of arrival in the diagnostic microbiology laboratory. Furthermore, if specific primers to amplify other flagellar antigen genes and other O antigen synthesis genes are designed, it might be possible to identify other human pathogenic Salmonella serovars by a combination of PCR primers responsible for O and H antigen genes using multiplex PCR.

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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