Conclusion

The GALIOSTM SNP genotyping technology is ideally suited for diagnostic SNP genotyping and has potential for high-throughput genotyping as well. The homogeneous assay principle and direct detection by single molecule fluorescence facilitate automation and minimize cross-contamination risks. Universal labeling primers provide a generic system for flexible, fast, and cost-efficient assay development without a need for the synthesis of labeled primers or specialty probes, and small reaction volumes reduce costs per bi-allelic SNP score effectively. The system is robust to variations in template concentration and provides highly sensitive and confident genotyping with as little as 1 ng of gDNA. High-content data from single molecule fluorescence provide intrinsic quality controls for highly reliable SNP scoring without additional control reactions.

Currently, by using FCS measurements, about 15,000 bi-allelic SNP scores per day can be analyzed on a single MF10 S instrument. Using Evotec Technologies' readers for ultra-high throughput screening (pHTS),[13] this number can be increased to at least 10-fold to allow for high throughput genotyping.

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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