Applications of oligonucleotides as PCR primers and microarray probes have expanded dramatically in recent years. The design of oligonucleotides for these applications involves a large number of considerations starting with the melting temperature and including a number of competing criteria. Each application has unique aspects making it impossible to cover the subject of oligonucleo-tide design in full generality. The key design issues are summarized in this article and discussed in terms of simple applications with reference to a software-based design approach that allows the user to balance the competing design criteria. This design approach has proven very successful but the final test of a well-designed oligonucleotide must be performed using the molecules themselves because biological systems exhibit more complex behavior than can be fully captured in software.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment