Conclusion

HyBeacon probes are fluorescent oligonucleotides that may be employed in real-time PCR assays to rapidly detect and discriminate nucleic acid sequences for a multitude of diagnostic applications. Probes emit higher levels of fluorescence when hybridized to target sequences than when single-stranded and permit target identification through melt peak analysis and Tm determination. The lack of probe secondary structure, quencher attachment, and sequence dependence renders HyBeacons simple and relatively inexpensive to design and synthesize. Multiple target sequences may be simultaneously analyzed using HyBeacon probes and assays typically take less than 20 min to complete. Saliva samples have been utilized directly in HyBeacon assays to further decrease analytical time and increase utility for point-of-care applications. HyBeacon assays are currently in development to detect pathogens in samples of urine and blood without sample purification.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment