Conclusion

At this time, bead-based nucleic acid testing might not be able to compete with high-density DNA arrays in gene expression profiling and with the determination of more precise expression levels using homogeneous real-time PCR methods. However, it has been shown that bead-based assays produce accurate results in low-density gene expression profiling and sequence detection. In terms of the analysis of sequence variations, especially genotyping of single nucleotide polymorphisms and point-mutations, bead-based technology provides a real alternative to conventional methods because of its flexibility and high multiplexing capability. The individual combination and the simultaneous determination of complete parameter profiles might be more cost-efficient than conventional single-analysis methods and so could find their way into laboratory diagnostics. Flow cytometry is a robust and automatable platform for bead-based testing. A large set of commercially available zip code beads (Luminex FlexMAP beads) provides an ideal condition for the development of new bead-based nucleic acid tests. With allele-specific primer extension (ASPE), an optimal wet-ware has been established, allowing high specificity, increased sensitivity, and thus requiring a less sophisticated platform at the same time.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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