Although multiplex real-time PCR can generally be described as the amplification and detection of multiple targets in a single reaction tube, there is tremendous variation with respect to the methods used to perform this technique and how the experimental data are utilized. By combining different available chemistries and different multiplexing strategies, the technique can be tailored for use in a number of different applications, from SNP analysis to multiple pathogen detection, and from oncology and molecular genetics to infectious disease uses. Presently, multiplex real-time technologies are limited only by the available instrumentation and chemistries, and it is currently practical to discriminate three to four targets in a single multiplex reaction. The combination of Tm determinations and multiple-colored flu-orophores gives the potential for the discrimination of many more targets, and this could be further expanded using technology such as microarrays. However, the expansion to microarrays and to greater degrees of multiplexing has yet to be achieved on a level that is satisfactory for routine clinical testing. Nevertheless, multiplex real-time methods that are currently available are already changing the diagnostic field, as they are providing more rapid and accurate results, and they are allowing scientists to answer clinical questions that were unable to be answered before the arrival of this technology.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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