There is no universally applicable ''ideal'' method for storing RNA. The procedure to be chosen is dependent on the source, the kind, and the intended use of the RNA. The most important factor that determines the quality of the stored RNA is the ability to protect the RNA from degradation by RNases. Therefore, if purified RNA is stored, RNase-free tubes and solutions are crucial, and the RNA should be stored frozen at temperatures as low as possible. Thawing and refreezing should be avoided.
If storage or shipment in a frozen state is not possible, samples from which RNA is to be extracted should be kept in a protein-denaturing agent to prevent RNase activity. For gene expression studies, any ongoing mRNA transcription activity should be suppressed as soon as possible after sample collection by snap-freezing the samples or by storage in an excess volume of RNA-stabilizing agent.
Detailed protocols for RNA extraction and storage can be found in molecular biology laboratory manuals.[12,13]
Was this article helpful?
The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.