We demonstrate here the technique of CISH on FFPE tissue sections. It has many advantages over traditional FISH. However, CISH also offer much more limited option than FISH in image analysis. The FISH signals can be computer enhanced, quantified, and analyzed. Computer-assisted analysis also allows multiple probes labeled with several different colors of fluorochrome to be used. Furthermore, the experimental procedure for FISH using directly labeled probe is less time consuming than CISH. However, in routine pathology, the convenience of CISH often outweighs its shortcoming. The most important advantage is that the morphology of tissue can be clearly visualized. They can then be compared with sections stained with other traditional methods, e.g., H+E and IHC, using conventional bright-field microscopy. The slides can also be archived as easily as other routinely prepared slides.
The combination of IHC with CISH, such as in the HER2 example, allows the confirmation of IHC results by ISH. Often, the CISH result was much more quantitative and less variable than the IHC data. More importantly, it allows the potential of examining gene modification and the consequence of protein expression on tissue section.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.