Conclusion

Differential display is a systematic, sensitive, and convenient approach that allows many samples to be tested in parallel, and does not require any previous knowledge of mRNA or gene sequence, making it an open system. Initially, DD analysis suffered from a high rate of false positives (or noise) due to the use of short primers and low annealing PCR temperature, and from redundancy in display because short primers may anneal to different parts of the same transcript. However, progressive technical improvements as well as care in experimental design have reduced these shortcomings. Because of its simplicity, sensitivity, reproducibility, and yield to automation, which increases throughput and accuracy, DD, either by itself or in combination with another expression profiling method, has become one of the most widely used methods for studying differentially regulated genes in many biological systems.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment