Fluorescence in situ hybridization analysis of human sperm cells has been used successfully in many studies: from studies of basic mechanisms of nondisjunction, to studies of infertility and effects of mutagens on the chromosome constitution of human sperm. The technique is simple, rapid, and relatively inexpensive. However, it is important to keep in mind that there are some subjective aspects to counting fluorescent signals and each laboratory must develop stringent scoring criteria for test subjects as well as laboratory-based control donors.
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