An increasing number of NASBA- and TMA-based assays—such as those for HIV-1 and HCV genomic RNA, CMV mRNA assays, as well as C. trachomatis and M. tuberculosis rRNA assays—containing all the neces sary components required for isolation, amplification, and detection have become commercially available. They are user-friendly and easy to use in clinical settings.

For various other applications, specific primers and probes have been designed that can be used in combination with the NucliSens Basic Kit, which contains all the components except for the specific primers and probes. These assays need further evaluation in clinical settings.

At present, ECL is the most sensitive method for the detection of the NASBA amplicon. An increasing number of real-time applications are being evaluated, even in multiplex formats. A major advantage of the use of molecular beacons is the ability to carry out real-time detection during amplification, which shortens the overall time of the assay, reduces the risk of contamination, and also provides information about the kinetics of the reaction.

Quantitative assays will become increasingly important, especially for the follow-up of patients, infected with blood-borne viruses such as HIV and HCV, who are under antiviral treatment.

Because NASBA and TMA have been shown to be unaffected by sensitivity and specificity problems associated with other isothermal techniques, it seems likely that their unique features will make this a technology that will be widely used in research and diagnosis. As the processes become more refined and standardized, both assays may displace or supplement current methods for rapid clinical laboratory diagnosis.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment