Gene expression profiling is a powerful tool for discovering genes and their products, and for generating new mechanistic hypotheses at the core of understanding the function of the genome. Approaches using SAGE share a number of strengths and limitations. Considering SAGE limitations, first, a small number of transcripts would be expected to lack an AE site and should be missed in the analysis. Although it is possible to utilize a second AE to overcome this problem, this has rarely been attempted in practice because of the effort and expense involved. Second, the approach relies on DNA sequencing, with its inherent cost and throughput limitations. Third, it is a statistical sampling method, which depends on the appropriate understanding and design of the study before the rigorous experimental part is even attempted.

A notable strength of the SAGE method, like no other gene expression profiling, is that results from any experiment are directly comparable to existing SAGE gene expression databases. Significant differences among these comparisons can be spotted by using a variety of statistical methods and bioinformatics to identify new transcriptomes.[21] Contrary to the static genome, an approach represented by the transcriptome is an interactive module that allows the study of the influence of external and internal factors, thereby serving as a dynamic link between an organism's genome and its physical characteristics.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment