as well as a real-time PCR for detecting the *5 null allele and duplication either by hybridization probes or by SYBR Green®. The null alleles *3, *4, *6, *7, and *8 were investigated by a conventional multiplex PCR but can also be carried out by real-time PCR. This again improves speed and is performed by use of two primer pairs and five different pairs of hybridization probes and subsequent fluorometric melting point analysis. Two separate real-time PCR reactions were performed in parallel: mutations *3 and *7 were analyzed in one capillary with two hybridization probes, one labeled with LightCycler-Red 640 and the other with LightCycler-Red
The CYP1A2 gene is not as polymorphic as the CYP2D6 gene. Twenty-five alleles have been described at present. It metabolizes psychoactive drugs such as antidepressants (e.g., amitriptyline, imipramine) and neuroleptics (e.g., clozapine) with variable activity which may lead to treatment resistance. In addition, it may play a role in carcinogenesis. Its expression is inducible by different environmental and dietary substances, such as tobacco smoking, as well as by several polymorphisms. Especially one polymorphism, a C to A transversion of base pair 734 in the noncoding intron 1, called *1F, is discussed to affect the inducibility of the CYP1A2 gene.[17,18]
Table 2 Sequences of the probes used for real-time PCR of CYP2D6
Sequence of probe 5'-3'
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.