Cytochrome P450 1a2

as well as a real-time PCR for detecting the *5 null allele and duplication either by hybridization probes or by SYBR GreenĀ®.[12] The null alleles *3, *4, *6, *7, and *8 were investigated by a conventional multiplex PCR[14] but can also be carried out by real-time PCR. This again improves speed and is performed by use of two primer pairs and five different pairs of hybridization probes and subsequent fluorometric melting point analysis.[15] Two separate real-time PCR reactions were performed in parallel: mutations *3 and *7 were analyzed in one capillary with two hybridization probes, one labeled with LightCycler-Red 640 and the other with LightCycler-Red

The CYP1A2 gene is not as polymorphic as the CYP2D6 gene. Twenty-five alleles have been described at present.[2] It metabolizes psychoactive drugs such as antidepressants (e.g., amitriptyline, imipramine) and neuroleptics (e.g., clozapine) with variable activity[3] which may lead to treatment resistance. In addition, it may play a role in carcinogenesis. Its expression is inducible by different environmental and dietary substances, such as tobacco smoking, as well as by several polymorphisms. Especially one polymorphism, a C to A transversion of base pair 734 in the noncoding intron 1, called *1F, is discussed to affect the inducibility of the CYP1A2 gene.[17,18]

Table 2 Sequences of the probes used for real-time PCR of CYP2D6

Detected allele

Sequence of probe 5'-3'

Reference

*2XN

GCACCCAATCCCAGCTAATTTTGTATT-Flu

LCRed640-TTGTAGAGACCGGGTTCTT-Ph

TGCTGCCTCCCACTCTGCAGTGCTC-Flu

LCRed640-ATGGCTGCTCAGTTGGACCCACGCT-Ph

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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