De novo Annotation

Example 1: from local discovery or newly published data to genome annotation.

In the lab, we sequenced several genes and discovered 100 SNPs. At that time (early 2002), public assembly of human gDNA sequence on 13q32-33 still had a lot of errors. We manually assembled a 17-Mb regional sequence with much better quality, which was later replicated by a subsequent public assembly. DNannotator was then used to annotate all 100 SNPs on this self-made sequence assembly. SNP data were prepared using Microsoft Excel spreadsheet and saved into a tab-delimited text file. DNannotator takes this SNP data file with gDNA sequence in FASTA format and maps all the SNPs to the self-made regional sequence. The Genbank format output is viewed in Artemis as shown in Fig. 2.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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