Because its composition is independent of the target being detected, the 5'-flap can be detected in a variety of ways, e.g., by size, sequence, charge, or fluorescence. Based on these properties, the Invader technology can be applied to numerous alternate detection formats including mass spectrometry, capillary electrophoresis, microfluidics, universal array chips, capture, eSensor™, fluorescence polarization, and time-release fluorescence.
ACLARA Biosciences (Mountain View, CA) produces small, electrophoretically distinct, fluorescent molecules called eTag reporter molecules that can be incorporated into primary probes as 5'-flaps. The released eTag flaps are resolved and quantitated using universal separation conditions on standard capillary DNA-sequencing instrumentation. High-level multiplex capabilities are possible with the development of hundreds of eTag reporters. ACLARA has demonstrated the ability to detect 26 Invader RNA assays from a single tube.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.