The TruGene™ HCV 5'NC Genotyping Kit. The
TruGene™ HCV 5'NC Genotyping Kit (Bayer Healthcare Diagnostics, Berkeley, CA) is based on a semiautomated terminator cycle sequencing technique of PCR products from the 5'UTR region conveniently retrieved from the Cobas Amplicor™ HCV Test (Roche Molecular Systems, Pleasanton, CA) or the Cobas AmplicorTM HCV Monitor Test (Roche). For the sequencing procedure, the CLIPTM sequencing technique (Bayer), allowing both directions of the amplification product to be sequenced simultaneously within the same tube, is employed. Electropho-resis and data analysis are carried out with the automated
Table 1 Methods for HCV genotyping
TruGene™ HCV 5'NC genotyping kit Celera HCV genotyping assay
Inno-LiPA HCV II (Versant)
HTA/HMA-Heteroduplex tracking/mobility assay
SIA-3 or SIA-6 RIBA HCV SIA
Celera; distributed by Abbott Innogenetics; distributed by Bayer Home-brew
Murex Diagnostics Chiron Corporation
DM; terminator cycle sequencing DM; real-time PCR
DM; restriction fragment length polymorphism DM; PCR and DNA enzyme immunoassay (EIA) DM; electrophoresis of hybridization products of RT-PCR products with ST-specific probes IM; serotyping IM; serotyping
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.