Direct Methods

Sequencing

The TruGene™ HCV 5'NC Genotyping Kit. The

TruGene™ HCV 5'NC Genotyping Kit (Bayer Healthcare Diagnostics, Berkeley, CA) is based on a semiautomated terminator cycle sequencing technique of PCR products from the 5'UTR region conveniently retrieved from the Cobas Amplicor™ HCV Test (Roche Molecular Systems, Pleasanton, CA) or the Cobas AmplicorTM HCV Monitor Test (Roche). For the sequencing procedure, the CLIPTM sequencing technique (Bayer), allowing both directions of the amplification product to be sequenced simultaneously within the same tube, is employed. Electropho-resis and data analysis are carried out with the automated

Table 1 Methods for HCV genotyping

Test name

Manufacturer

Method

Gen. region

TruGene™ HCV 5'NC genotyping kit Celera HCV genotyping assay

Inno-LiPA HCV II (Versant)

RFLP analysis

Gen-Eti-K DEIA

HTA/HMA-Heteroduplex tracking/mobility assay

SIA-3 or SIA-6 RIBA HCV SIA

Bayer

Celera; distributed by Abbott Innogenetics; distributed by Bayer Home-brew

Sorin Biomedica

Home-brew

Murex Diagnostics Chiron Corporation

DM; terminator cycle sequencing DM; real-time PCR

DM; reverse-hybridization

DM; restriction fragment length polymorphism DM; PCR and DNA enzyme immunoassay (EIA) DM; electrophoresis of hybridization products of RT-PCR products with ST-specific probes IM; serotyping IM; serotyping

5'UTR

5'UTR

5'UTR

5'UTR

Core

Core/E1

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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