Dna Detection

The target-specific region of the primary probe is designed to have a melting temperature close to the reaction temperature, typically 63°C. Its inherent instability allows for the exchange of cleaved and uncleaved probes onto the target. When the specific sequence is present, the Invader oligo and TSR of the primary probe form an invasive structure on the target. The Cleavase enzyme removes the 5'-flap plus position 1 of the target-specific region. Following cleavage, the primary probe dissociates from the target and is replaced with another uncleaved primary probe, which is present in excess. Thus numerous 5'-flaps are released for each target molecule present, resulting in a linear amplification of signal, but not target.

The cleaved 5'-flap then acts as an Invader oligo to generate another invasive structure with a synthetic molecule, the fluorescence resonance energy transfer (FRET) oligo, which contains a donor fluorophore on the overlapping base (position 1) and a quencher fluorophore on the other side of the cleavage site.

DNA Invader® assay

Primary Reaction

5'. ^cleavage site

INVADER® oligo 51

5'. ^cleavage site primary probe 3'

primary probe 3'

Secondary Reaction

DNA target cleavage site cleaved 5'-flap gaBSSB-S-l

FRET probe

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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