Dnabased Identification Ligase Chain Reaction

A ligase chain reaction (LCR) assay with the thermostable Thermus aquaticus DNA ligase has been devised for the specific detection of Listeria monocytogenes. This assay is based on a single base-pair difference in the V9 region of the 16S sequence of the genes coding for rRNA, which distinguishes L. monocytogenes from other closely related Listeria spp. To achieve a higher sensitivity, the 16S rRNA gene was initially amplified by PCR prior to LCR. The LCR was originally performed with radioactive-labeled primers, and ligation products were detected by autoradiography after denaturing gel electrophoresis (DGGE). The method was further adapted for the detection of LCR products with a nonisotopic readout using chemiluminescent or colorimetric substrates. The latter method gave a sensitivity of 10 CFU of L. monocytogenes and is amenable to automation.1-1,2-1

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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